Uric acid elimination using honeycomb carbon support derived from rice husk and lignin

One of the promising research area, which today is particular importance in biomedicine, use enzyme surface modified honeycomb carbon supports for biocatalysis and haemosorption. Patients suffering from hyperuricemia, with elevated blood levels urates are prone to diseases such as gout, chronic kidney disease, nephrolithiasis and, finally, an acute renal failure. In this work, monoliths were produced via extrusion milled rice husk organosolv lignin mixture at a mass ratio 75:25, followed by carbonization, disilication, successive steam-gas activation. Morphology textural characteristics analyzed means scanning electron microscopy low-temperature nitrogen adsorption porosymetry, e.g.: nanoporosity activated samples was established; specific area according BET-method 1323 m2/g, total pore volume DFT model 1.053 cm3/g. The uricase immobilized on amount 1 unit per 1.5 g support using cross-linking agent N-cyclohexyl-N′-(2-morpholinoethyl) carbodiimide methyl-p-toluenesulfonate. order elucidate whether sodium urate substrate removal assistance derived takes place due and/or catalysis, investigation conducted recirculatory system, both inert argon atmosphere, oxidizing atmosphere presence oxygen room temperature. It established that able eliminate up 92% uric acid solution concentration 66 mg/L, minimal leaching enzyme, entry into body can lead manifestation allergy. substantiated number units be minimized optimizing procedure immobilizing support.